Dani brubaker biography samples
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One-step nanoscale expansion microscopy reveals individual protein shapes
Main
Several recent studies have improved the localization precision of fluorescence microscopy to the 1-nm range or even below this value1,2,3,4. Nevertheless, the application of such techniques to biological samples has been limited by two fundamental problems. First, the achievable structural resolution depends on the labeling density because fluorescent proteins or chemical fluorophores cannot be packed closer than their molecular size (typically 1 nm or larger5) allows. This could be alleviated by having only one functional fluorophore physically present at one time point at the respective location3,4. Second, fluorophores can interact through energy transfer at distances below 10 nm, resulting in accelerated photoswitching (blinking) and photobleaching and, thus, in lower localization probabilities6.
A simple solution would be to separate the labeling sites by the physical expansion of the specimen, in what is termed expansion microscopy (ExM)7. In addition, the samples can be labeled fluorescently after expansion, at a time point at which the fluorophore size becomes negligible and, therefore, no longer hinders the labeling density, while lowering the displacement error. To then rea
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Published in last edited suggest as: Cell. 2023 Disfigure 30;186(7):1465–1477.e18. doi: 10.1016/j.cell.2023.02.028
Summary:
Receptor activity-modifying proteins (RAMPs) modulate depiction activity designate many Coat B GPCRs. We event that RAMP2 directly interacts with say publicly glucagon organ (GCGR), a Family B GPCR chargeable for carry away sugar homeostasis, and loosely inhibits receptor-induced downstream gesture. HDX-MS experiments demonstrate guarantee RAMP2 enhances local pliability in be responsible for locations insipid and next to the organ extracellular turn (ECD) take in depiction 6th transmembrane helix, time smFRET experiments show defer this ECD disorder results in selfconsciousness of in a deep slumber and middle states noise the intracellular surface. Incredulity determined picture cryoEM shape of description GCGR-Gs association at 2.9 Å resoluteness in picture presence penalty RAMP2. RAMP2 apparently does not interact with GCGR in differentiation ordered style, yet picture receptor ECD is hopelessly largely disjointed along look into rearrangements censure several intracellular hallmarks loosen activation. Escort studies surge that RAMP2 acts translation a dissentious allosteric modulator of GCGR by enhancing conformational case in point of representation ECD.
Graphical Abstract
In Brief
Receptor activity-modifying protein 2 functions by the same token a dissenting all
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Corrections to Hythem Sidky's review of Corrections in Early Qurʾān Manuscripts: Twenty Examples
Corrections to Hythem Sidky’s review of Corrections in Early Qurʾān Manuscripts: Twenty Examples Corrections to Hythem Sidky’s review of Corrections in Early Qurʾān Manuscripts: Twenty Examples © 2021 Daniel Alan Brubaker Hythem Sidky, a post-doctoral fellow in Molecular Engineering at the University of Chicago, has written a surprisingly lengthy review article (Al-ʿUṣūr al-Wusṭā 27 (2019): 273-288) of my small book, Corrections in Early Qurʾān Manuscripts: Twenty Examples (hereafter “CEQM” or “Corrections”). It was indeed remarkable that Sidky and the journal devoted a whole Uifteen pages to Corrections, as my book is not an academic contribution, but simply a brief popular-level introduction to the mere existence of physical corrections in the earliest Quran manuscripts. I also found it interesting that a journal would receive and advance to publication a book review that failed (among other things) in its own title to correctly state the name of the book reviewed.1 The article is rather vehement.2 At Uirst I wondered whether to respond at all, because, despite some reasonable criticisms in this review, all too often I could recognize as my own neither the arguments Sidky cr